UNIVERSITY of the LAGOON
PROGRAM OF THE THIRD CYCLE
BIENNIUM 1999-2001
SCIENCES OF THE LIFE AND THE ENVIRONMENT
INTERDEPARTMENTAL PROGRAM
MACRO-MOLECULE CHARACTERIZATION
*Dep. Chemical engineering and Pharmaceutical Technology
Credits: 6
Date of teaching: From the 12 to the 30 of June.
Schedule: Theory, from 15,30 to 18.30. Practices, from 9 to 14.00.
Classroom: Laboratory of Galénica.Facultad Pharmacy of Pharmacy
Professors:
THEORY.
1.- Types of macro-molecules.
1,1 Biodegradable synthetic polymers: Methods of obtaining, biomedical characteristics and applications.
1,2 Peptides and proteins: Covalentes and three-dimensional structures, properties in dissolution, interactions with other molecules.
2.- Determination of the molecular weight.
2.1. Foundations of HPLC: Instrumentation, columns, mechanisms of separation, analysis and data processing.
2.2. Chromatography of exclusion (ESA, GFC and GPC): Foundations, average distribution of weights and molecular weights.
2.3. Detectors: Refractometers differentials, viscometers, diffraction of light laser multiangle, combined. Relative and universal calibration. Absolute determination of the p.m.
3.- Thermal Characterization: Calorimetry of Sweeping Differential.
3.1. Foundations.
3.2. Instrumentation: DSC of compensated power. DSC of heat flow.
3.3. Applications.
4.- Thermal polymer Characterization.
4.1. Vitreous transition temperature.
4.2. Methods of determination of the vitreous Transition temperature.
5.- Thermal protein Characterization.
5.1. Protein stability: Physical instability and chemical instability.
5.2. Reversible and irreversible transitions.
5.3. Techniques of stabilization: Physical methods. Excipientes.
6.- Structural Characterization.
6.1. RMN of 1H.
6.2. RMN of 13C.
YOU PRACTICE OF LABORATORY.
1.- Biodegradable polymer Characterization derived from lactic acid (PLA).
1,1 Calibration of the GPC system using polystyrene landlords.
1,2 Preparation and analysis of PLA samples.
1,3 Determination of the p.m. average relative.
1,4 Determination of p.m. absolute using double detection (diffraction and Refractive index of light laser multiangle).
2.- In the middle watery protein Analysis and quantification.
2,1 Obtaining of a calibrated straight line of for the albumen determination by HPLC-RP.
2,2 Calibration of a system of HPLC-SE for the determination of protein p.m. relative.
2,3 Determination by absolute HPLC-SE of p.m. using a system of triple detection (UV, TO GO and MALLS).
3.- Thermal Characterization of biodegradable polymers and proteins.
3,1 Determination by DSC of the vitreous transition temperatures of poliláctico acid polymers and poliláctico-coglicólico acid copolymers.
3,2 Determination of the temperatures of globular protein denaturation by DSC.
COMPARTMENTAL ANALYSIS
*Dep. Chemical engineering and Pharmaceutical Technology
Credits: 3
Date final beginning/24 April - 12 of May 2000
Classroom: Laboratory of Galénica Pharmacy. Faculty of Pharmacy
Professors:
Subject 1. Introduction to the compartmental models
- Introduction
- Objectives
- Models and systems
- Classification of the compartmental models
- Noncompartmental compartmental models
- Physiological models
- Systems in nonstationary state and stationary state
- Compartment
- Compartment nonsaturable and saturable
- Kinetic of transference and transformation
- Intercompartmental explanation
- Construction of the pharmacokinetic models
- Analysis, simulation and studies of sensitivity in pharmacokinetics
Subject 2. Methods of resolution of the linear models
- Introduction
- Differential equations
- Resolution of differential equations by means of the transformation of Laplace
- Transformed of Laplace
- Resolution of systems of linear differential equations
- Convolución
Subject 3. Linear models of 1 compartment
- Introduction
- Models in stationary state
- Models in nonstationary state
- Metabolic transformation
Subject 4. 2 linear models of compartment
- Introduction
- Two models of compartment with function of entrance d Dirac
- Identificabilidad of the model
- Two models of compartment with function of stepped entrance
- Two models of compartment with function of exponential entrance
Subject 5. Simulation. Part I
- Introduction to the Mathematica program ®
- Graphical functions
- Representation of functions
- Representation of lists
- Combined graphs of functions and lists
- Description of the Graphics package
Subject 6. Simulation. Part II
- Resolution of systems of differential equations.
- DSolve function []
- Numerical resolution of systems of differential equations
- NDSolve function []
- Method of Runge Kutta
- Description of the RungeKutta package
Subject 7. Models mamilares
- Introduction
- Models mamilares with function of entrance d of Dirac
- Identificabilidad and estimation of parameters
- Models mamilares with function of stepped entrance
- Models mamilares with function of exponential entrance
- Determination of the entrance function
- Applications
Subject 8. Models nonlinear
- Introduction
- Models with saturable elimination
- Kinetic of Michaelis - They mention
- Saturable models compartment
- Simulation of models nonlinear
Subject 9. Analysis of the drug absorption
- Introduction
- Classification of the methods
- Methods based on the material balance
- Methods based on the deconvolución
- Numerical Deconvolución
Subject 10. Estimation of parameters
- Linear regression
- Regression nonlinear
- Foundations
- NonlinearFit function []
- Analysis of the residues
- Selection of models
USE OF RADIOACTIVE TRACERS IN INVESTIGATION
*Dep. Chemical engineering and Pharmaceutical Technology
Credits: 3 (16 hours theoretical and 14 practices)
Date of final beginning/: 17 to 28 of January of 2000
Classroom: Laboratory of Galénica Pharmacy and Isotope Laboratory
Faculty of Pharmacy
Professors:
Subject 1. Radioactivity.
Stable and unstable centres. Natural radioactivity and artificial. Disintegration constant. Period of half-life. Average life. Radioactive equilibrium. Types of nuclear reactions. Production of radioactive nuclides.
Subject 2. Radioactive disintegration.
Emission alpha. Emission beta. Gamma-ray emission. Positron emission. Phantoms and disintegration energy. Schemes of disintegration. Laws of radioactive disintegration.
Subject 3. Synthesis of radioactive tracers.
Techniques. Marking with more habitual radioactive nuclides. Purification. Degradation of radioactive tracers.
Subject 4. Particle interaction with the matter.
Types of collision. Stopping power and reaches. Specific ionization.
Phenomena of ionization and activation. Interaction of the alpha radiation, beta, gamma, positrons and neutrons.
Subject 5. Radiological magnitudes and units.
Activity. Exhibition. Absorbed dose. Biological effectiveness. Dose equivalent. Dosimetry of the radiation. Units and systems.
Subject 6. Radioactivity indicators.
Physical foundations of the detection. Photographic detectors. Detectors of ionization. Solid and liquid scintillation detectors. Semiconductor detectors. Accountants. Other equipment.
Subject 7. Introduction to the Radiological safety.
Concept and objectives. Effect of radiation on the alive matter. Basic principles of PR. Dosimetry. Irradiation and contamination. Dose and limit. Operational radiation protection.
Subject 8. Use of radioactive tracers in Investigation.
Basic slight knowledge of tracers. Analytical techniques. Use in Biochemistry. Other uses.
YOU PRACTICE
1. Radiological safety: Basic principles. Monitors. Dosimetry.
2. Contaje gamma. Accountants, phantoms, contaje with double isotope.
3. Contaje beta. Liquid scintillation counters.
4. Radioimmunoassay.
NORMS OF GOOD PRACTICES OF LABORATORY
*Dep. Chemical engineering and Pharmaceutical Technology
Credits: 3
Date of final beginning/8-19 November 1999
Classroom: Laboratory of Galénica Pharmacy. Faculty of Pharmacy.
Professors:
Objective: Knowledge of the norms BPL that must be followed by the laboratories credited in the nonclinical tests of substances and chemical agents in agreement with the Spanish legislation.
Program
Subject 1.- Principles of good practices of laboratory.
- Objective and concepts related to the organization of a laboratory.
- Organization and personnel of laboratory: responsibilities of the direction and responsibilities of the personnel
Subject 2.- Program of quality assurance
- Majorities
- Responsibilities of the personnel.
- Terms regarding the study and to the substance to try.
Subject 3.- Requirements of the laboratories
- Facilities related to the experimental system and the handling of the substances to try and reference.
- Rooms of archives.
- Waste disposal.
Subject 4.- Experimental Systems
- Physico-chemical systems.
- Substances to try and of reference.
- Validation of analytical methods.
- Biological systems.
- Environmental conditions in the rooms of the estabulario, control and registry.
- Reception, lodging and group of forty.
- Contribution of water and food to the animal.
Subject 5.- Standard Procedures of work
- Applications: pipettes, balance, pH-meter, spectrophotometer.
Subject 6.- Accomplishment of studies and closing report
- Protocols.
- Presentation of results
- Storage and conservation of archives.
Subject 7.- Inspection